2007 International Mock Board Exam Coalition

Central Region	University of Texas
Southwestern Medical Center	03/04/2007	
Northeastern Region	Yale University
	03/24/2007	
Centers for Disease Control and Prevention	Center for Disease Control and Prevention, Atlanta, GA	04/13/2007	
Southeastern Region	North Carolina State University
	05/19/2007	
International
	Europe	06/02/2007	
Pacific Northwest Region	Oregon Health & Science University
and Portland VA Medical Center	06/09/2007	

Practical Section – 75 Questions
Referenced Answers

27 pages

Practical Exam Contributors
Cheryl Haughton, DVM, DACLAM - Central Region Coordinator
David Kurtz, DVM, PhD, DACLAM – Southeastern Region Coordinator
Stephanie Murphy, VMD, PhD, DACLAM - Pacific Northwest Region Coordinator
Peter Smith, DVM, DACLAM - Northeastern Region Coordinator
Mallory Tate, DVM – CDCP Coordinator
Mary Ann Vasbinder, DVM, DACLAM - Southeastern Region Coordinator
Scout Chou, VMD, DACLAM
Suzanne Craig, DVM, DACLAM
Sylvia Gografe, DVM, PhD, DACLAM
Angela King-Herbert, DVM, DACLAM
Ken Salleng, DVM

This examination is meant to be used as a study tool when preparing for the ACLAM Certifying Examination.  The material presented in this mock examination follows the role delineation document, but is not necessarily reflective of the ACLAM Certifying Examination.

There is one correct answer for each question.

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Etiology, etiologic agent, cause or causative agent:  Indicate only the agent, e.g., Mycobacterium tuberculosis or trauma

Etiologic diagnosis:  Indicate the causative agent and the site of the lesion, e.g., esophageal candidiasis

Morphological diagnosis:  Indicate the organ, the process and the distribution (if indicated), e.g., diffuse ulcerative colitis or uterine Adenocarcinoma.

Disease:  Indicate the common name for the condition, e.g., measles or slobbers

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1.	What is the function of the piece of equipment shown in the slide?

a.	Metabolic chair
b.	Restraint device
c.	Surgical device
d.	Supply storage
e.	Feeding device

Answer: b, restraint device
Reference:  http://www.primateproducts.com/home.php?cat=11; Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 719.
Task:  3K1
Species:  Primary (nonhuman primate; macaque)

2.	What is the genus and species of this rabbit parasite?

a.	Chyletiella parasitovorax
b.	Psoroptes cuniculi
c.	Listrophorus gibbus
d.	Sarcoptes scabiei
e.	Passalurus ambiguus

Answer: c.  Listrophorus gibbus
Reference: Ectoparasites of  Rodents and Rabbits; www.radil.missouri.edu 
Task:  1K3
Species:  Primary (rabbit)

3.	What is the condition depicted in this slide?

a.	Genital wart
b.	Genital hernia
c.	Dystocia
d.	Rectal prolapse
e.	Prolapsed prepuce

Answer:  d.  Rectal prolapse
Reference:  Harkness, J. and Wagner, J.  1995.  The Biology and Medicine of Rabbits and Rodents.  Williams and Wilkins, Media, PA p. 163
Task:  1K8
Species:  Primary (mouse)

4.	Which agent is likely to cause the condition in these slides?

a.	Swine pox
b.	Pediculosis
c.	Sarcoptes scabiei
d.	Dermatophytosis
e.	Herpesvirus

Answer:  c.  Sarcoptes scabiei
Reference:    Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 656.
Task:  1K3
Species:  Primary (pig)

5.	Which type of suture material is most likely to cause this type of skin lesion in Xenopus laevis?

a.	Monofilament nylon
b.	Braided silk
c.	Polypropylene
d.	Polyglactin 910
e.	Polydioxanone

Answer: b. braided silk
References:  Tuttle A. D., MacLaw J., Harms C. A., Lewbart G. A., and Harvey S. B. 2006. Evaluation of the gross and histologic reactions to five commonly used suture materials in the skin of the African clawed frog (Xenopus laevis). JAALAS. 45:22-26.
Task:  T3K1
Species:  Secondary (African clawed frog)

6.	During a surgical procedure the femoral artery of a rabbit was ligated to induce ischemia. What is the instrument in the lower part of the picture called to spread the incision?

a.	Senn Rake Retractor
b.	Weitlander Retractor
c.	Balfour Retractor
d.	Gelpi Retractor
e.	U.S. Army-Pattern Retractor

Answer: b.  Weitlander Retractor
Reference: Sonsthagen, T.F. 2006. Veterinary Instruments and Equipment: A Pocket Guide. p. 535-545. Elsevier Mosby, Inc., Philadelphia, PA.
Task:  T3K1
Species:  Primary (rabbit)

7.	What is the most likely name of the disease causing the condition depicted in these three pictures?

a.	Hydronephrosis
b.	Spontaneous chronic progressive nephrophathy
c.	Urinary calculi
d.	Proteus mirabilis
e.	Mouse urologic syndrome

Answer: e.  Mouse urologic syndrome
Reference: Percy, D.H. and Barthold, S.W. 2001. Pathology of Laboratory Rodents & Rabbits. p. 92-95. Iowa State University Press, Ames, Iowa.
Task:  T1K5
Species:  Primary (mouse)

8.	What is device depicted in this slide called?

a.	Skinner box
b.	Holtzman box
c.	Induction chamber
d.	Metabolic cage
e.	Barnes Maze

Answer:  a, Skinner box
Reference:  http://en.wikipedia.org/wiki/Skinner_box 
Task:  T9K5
Species:  Primary (rat)

9.	What is the genetic designation for this immunodeficient mouse?

a.	hr-hairless
b.	hrrh-hairless rhino
c.	nu-nude
d.	nustr-nude streaker
e.	motheaten

Answer:  b.  hrrh-hairless rhino
Reference:  http://jaxmice.jax.org/library/notes/436c.html 
Task:  T3K3
Species:  Primary (mouse)

10.	Which of the following organism is reported to contribute to this clinical condition seen in gerbils?

a.	Staphylococcus xylosus
b.	Staphylococcus intermedius
c.	Streptococcus pneumoniae
d.	Staphylococcus meriones
e.	Bordetella bronchiseptica

Answer:  a.  Staphylococcus xylosus
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 277.
Task:  T1K8
Species:  Secondary (gerbil)

11.	Which is the method of choice to euthanize this species?

a.	Decapitation
b. 	Carbon dioxide asphyxiation
c. 	IV barbiturate
d. 	Electrocution
e.	Tricaine methane sulfonate in water 

Answer:  e.  Tricaine methane sulfonate in water
Reference:  Beaver, B., et.al.  2001.  “2000 Report on the AVMA Panel on Euthanasia.”  JAVMA 218 (5) p. 693.
Task:  T2K5
Species:  Secondary (African claw toed frog)

12.	Unlike Old World primates, which of the following is an essential nutrient for this species?

a.	Vit. B6
b.	Vit. B12
c.	Vit. D3
d.	a and c
e.	none of the above

Answer:  c.  Vit. D3
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 685.
Task:  T1K16
Species:  Tertiary (other nonhuman primates)

13.	According to the Animal Welfare Act, what group is this cetacean (Delphinus delphis) placed for minimum horizontal dimension (MHD)?

a.	I
b.	II
c.	III
d.	IV
e.	V

Answer:  b.  II
Reference:  Animal Welfare Act, 9 CFR Part 3 – Standards, Subpart E – Specifications for the Humane Handling, Care, Treatment, and Transportation of Marine Mammals, 3.104 Space Requirements, p. 50.
Task:  T5K1
Species:  Tertiary (other mammals)

14.	This finding was detected in a pig at necropsy.  How should this finding be interpreted?

a.	Abnormal
b.	Only seen in this species
c.	Malignant tumors
d.	Sporadic incidental finding

Answer:  d.  Sporadic incidental finding 
Reference:  1998 .  Merck Veterinary Manual 8th ed.   Merck & Co., Rahway, N.J.  p. 1005.
Task:  T1K5
Species:  Primary (pig)

15.	The 3-week to 4-month-old pigs shown in this slide presented for lameness, depression, neurological signs, and death.  Gross necropsy findings included polyarthritis, fibrinous pleuritis and pericarditis, and leptomeningitis.  Of this list, what is the most likely etiological agent?  

a.	Haemophilus parasuis
b.	Brachyspira hyodysenteriae
c.	Mycoplasma hyopneumoniae
d.	Wilsonia stevenicus
e.	Pasteurella multocida

Answer:  a.  Haemophilus parasuis
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 203-247.
Task:  T1K8
Species:  Primary (pig)

16.	What is the most reliable characteristic distinguishing adult males from females of the genera shown in this slide?

a.	Anogenital distance
b.	Only the males produce alkaloid toxin
c.	The males are brightly colored, while the females are a dilute brown color
d.	In males, the tympanic membrane is twice the size of females
e.	The females are mute

Answer: e.  The females are mute
Reference: St. Claire MB, MJ Kennett, ML Thomas, and JW Daly. 2005. “The Husbandry and Care of Dendrobatid Frogs.” Contemporary Topics in Laboratory Animal Science 44(6): 8-14 
Task:  T4K1
Species:  Tertiary (other amphibians)

17.	Upon necropsy of a recently acquired cynomolgus macaque the following lesion was noticed.  What is the etiology for this condition?

a.	Strongyloidosis
b.	Nochtia nochti
c.	Hydatid cyst
d.	Cysticercosis
e.	Pentastomes

Answer:  e. Pentastomes
Reference:  Flynn RJ.  1973.  Parasites of Laboratory Animals.  Iowa State University Press.  pp. 495-498.
Task:  T1K3
Species:  Primary (macaque)

18.	What is the use of this device in laboratory animal facilities?

a.	Air purification
b.	Sound attenuation
c.	Water purification
d.	Protein analysis
e.	Waste disposal

Answer:  c.  water purification, reverse osmosis water purification
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 368; http://www.edstrom.com/products_ro.cfm?doc_id=322
Task:  T6K9
Species:  None.

19.	When this animal is fed an arginine free diet and exposed to influenza and aspirin, what human disease is induced?  

a.	H5N1
b.	1812 Influenza virus
c.	Hypergammaglobulinemia
d.	Reyes syndrome
e.	Sjogren’s syndrome

Answer:  d.  Reyes syndrome
Reference:  Fox, JG.  1998.  Biology and Diseases of the Ferret, 2nd edition.  Williams & Williams, p. 133.
Task:  T9K1
Species:  Secondary (ferrets)

20.	What is the genus of this animal?

a.	Mastromys
b.	Peromycus
c.	Mystromys
d.	Spermophilus
e.	Sigmodon

Answer:  e.  Sigmodon
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 270.
Task:  T3K3
Species:  Tertiary (other rodents)

21.	What is this species a common model for?

a.  Adenovirus
b.  Plague
c.  Measles
d.  Leishmaniasis
e.  Herpesvirus

Answer:  a.  Adenovirus
Reference:Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 270.  
Task:  T3K2
Species:  Tertiary (other rodents)

22.	This is a fluid percussion device, it is most commonly used to

a.	Test material hardness
b.	Drill bones
c.	Create dental anomalies
d.	Induce neurologic lesions
e.	Test auditory function

Answer:  d.  induce neurologic lesions
Reference:  http://www.dragonflyinc.com/fluid_perc.htm 
Task:  T3K1
Species:  None

23.	This is an F/air canister.  The NIOSH maximum limit for personnel exposure to halogenated anesthetic gases is:

a.	2 ppm
b.	8 ppm
c.	20 ppm
d.	40 ppm
e.	60 ppm

Answer: a.  2 ppm
Reference:  http://www.cdc.gov/niosh/pdfs/77-140a.pdf
Task:  T2K6
Species:  None

24.	What is the important type of enrichment needed for this animal?

a.  Secluded areas
b.  Individual housing
c.  Dust baths
d.  Light box
e.  Whole body feeder

Answer:   c.  Dust Baths
Reference:  Brown, SA and Rosenthal, KL.  1997.   Self Assessment Color Review of Small Mammals.  Iowa State Press, Ames, IA, p. 26.
Task:  T4K1
Species:  Tertiary (other rodents)

25.	What must be done to ensure the device functions well that the employee is wearing?

a.  Fit Testing
b.  Culturing
c.  Health screen
d.  Body wraps
e.  Body  acclimate

Answer:  a.  Fit testing
Reference:  Committee on Occupational Safety and Health in Research Animal Facilities, Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council.  1997.  Occupational Health and Safety in the Care and Use of Research Animals.  National Academy Press, DC.  Chapter 4 – Allergens, p. 63. 
Task:  T6K3
Species:  None.

26.	What does the document depicted on this slide state with respect to training?

a.  Only animal care staff need be trained to work with animals.
b.  Technician staff may not train investigators in animal use.
c.  Investigators, techs, etc. must be trained to do animal techniques.
d.  Formal training is not necessary to work with animals.

Answer:  c.  Investigators, techs, etc. must be trained to do animal techniques.
Reference:  Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council.  1996.  Guide for the Care and Use of Laboratory Animals.  Chapter 1 Institutional Policies and Responsibilities, p. 13.
Task:  T8K1
Species:  None

27.	What is the genus and species of this parasite found in rodents?

a.	Syphacia muris
b.	Syphacia obvelata
c.	Rodentolepis nana
d.	Aspicularis tetraptera
e.	Hymenolepsis diminuta

Answer:  c.  Rodentolepis nana
Reference:  http://www.radil.missouri.edu/info/dora/mousepag/zoon.html 
Task:  T4K9
Species:  Primary (mouse)

28.	What is the purpose of the depicted apparatus?

a.	Feeding
b.	Port implantation
c.	Restraint
d.	Orthopedic repair

Answer:  c.  Restraint
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 719.
Task:  T5K2
Species:  None (General non-human primate)

29.	What is the objective of AWIC?

a.  Provide an incident command in the case of animal emergencies 
b.  Report animal welfare concerns to the USDA
c.  A CDC directive to inform the public about Avian Influenza
d.  Provide information on animal welfare

Answer:  d.  Provide information on animal welfare
Reference:  http://awic.nal.usda.gov/nal_display/index.php?tax_level=1&info_center=3 
Task:  T7K1
Species:  None

30.	What is the advantage of the dual corridor system in animal facility design?

a.	Easy movement in the animal facility
b.	To decrease the potential for cross contamination 
c.	Optimized animal housing space
d.	Decreased cost of building the facility.

Answer:  b.  To decrease the potential for cross contamination
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 912.
Task:  T1K3
Species:  None

31.	What is the purpose of this apparatus?

a.	Communication
b.	Vascular access
c.	Electrical stimulation
d.	Surveillance

Answer:  b.  Vascular access
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 1013.
Task:  T5K2
Species:  None

32.	The wound depicted in this slide was inflicted by the animal depicted in this slide.  What is the zoonotic pathogen of concern?

a.	Streptobacillus moniliformis
b.	Brucella bovis
c.	Toxoplasma gondii
d.	Bartonella henselae

Answer:  d.  Bartonella henselae
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 1079.
Task:  T4K9
Species:  Secondary (cat)

33.	List the genus and species of the animal depicted in this slide:

a.  Chinchilla laniger
b.  Chinchilla chinchilla
c.  Oryctolagus cuniculus
d.  Mesocricetus auratus
e.  Cavia porcellus

Answer:  a.  Chinchilla laniger
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 286.
Task:  T3K3
Species:  Tertiary (other rodents)

34.	Name the most likely cause of the condition of these mice.

a.  Barbering
b.  Tail vein bleeding
c.  Fight / bite wounds
d.  Foot pad injections
e.  Ectromelia

Answer:  c.  Fight / bite wounds
Reference:  Harkness, J. and Wagner, J.  1995.  The Biology and Medicine of Rabbits and Rodents.  Williams and Wilkins, Media, PA p. 163.
Task:  T1K8
Species:  Primary (mouse)

35.	Identify the equipment depicted in this slide:

a.  Anesthesia machine
b.  Fluid pump
c.  Blood gas machine
d.  Cautery
e.  Blood pressure monitor

Answer:  b.  Fluid pump
Reference:  http://www.pennvet.com/content/equipmentcatalog/fluid_pumps.asp
Task:  T6K10
Species:  None

36.	Name the condition of this mouse.

a.  Scabby skin disease
b.  Scaly skin disease
c.  Mouse mange
d.  Skin slough
e.  Ulcerative dermatitis

Answer:  e.  Ulcerative dermatitis
Reference:  Percy, DH and Barthold, SW.  2001.  Pathology of Laboratory Rodents and Rabbits.  Iowa State Press, Ames, IA, p. 75.
Task:  T1K8
Species:  Primary (mouse)

37.	According to the Animal Welfare Act, what minimum age must this animal be to be transported in the same primary enclosure as adults, other than its dam?

a.  12 weeks
b.  6 months
c.  1 year
d.  4 months
e.  8 weeks

Answer:  d.  4 months
Reference:  Animal Welfare Act, 9 CFR Part 3 – Standards, Subpart A – Specifications for the Humane Handling, Care, Treatment, and Transportation of Dogs and Cats, 3.7 Compatible Grouping, p. 50.
Task:  T7K1
Species:  Primary (dog)

38.	According to the CDC, which Biosafety level(s) requires this type of containment?

a.  BSL-1
b.  BSL-1 & BSL-2
c.  BSL-3
d.  BSL-2 & BSL-3
e.  BSL-3 & BSL-4

Answer:  d.  BSL-2 & BSL-3
Reference:  CDC – NIH 1999.  Biosafety in microbiological and Biomedical Laboratories, 4th ed.  U.S. Dept. of Health and Human Services, Washington, D.C. p. 205 (Table 1, appendix A)
Task:  T7K1
Species:  None

39.	Name the device.

a.  Rodent microwave
b.  Sterotaxic device
c.  Glass bead sterilizer
d.  Instrument holder
e.  Micro autoclave

Answer:  c.  Glass bead sterilizer
Reference:  http://www.sterilizers.com/glass_bead/
Task:  T4K2
Species:  None

40.	What does this acronym stand for?

a.  Certified Mouse Animal Researcher
b.  Certified Murine Animal Researcher
c.  Certified Manager of Animal Resources
d.  Certified Manager of Animal Research
e.  Certified Manager of Laboratory Animal Resources

Answer:  c.  Certified Manager of Animal Resources
Reference:  http://cob.jmu.edu/icpm/CMAR_program.htm
Task:  T8K2
Species:  None

41.	The following level of lighting is recommended by the Guide:

a.	400 lux at 3 meters above the floor
b.	325 lux at 2 meters above the floor
c.	325 lux at 1 meter above the floor
d.	425 lux at 1 meter above the floor

Answer:  c.  325 lux at 1 meter above the floor
Reference:  Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council.  1996.  Guide for the Care and Use of Laboratory Animals.  Chapter 1 Institutional Policies and Responsibilities, p. 35
Task:  T6K4
Species:  None

42.	Which of the following drugs is recommended when malignant hyperthermia becomes an anesthetic complication?

a.	Fluids only
b.	Flunixin megalamine
c.	Dantrolene
d.	Pentobarbitol

Answer:  c.  Dantrolene
Reference:  Kohn, DF, Wixson, SK, White, WJ, and Benson, GJ.  1997.   Anesthesia and Analgesia in Laboratory Animals.  Academic Press, New York, NY.   p. 325.
Task:  T1K6
Species:  Primary (pig)

43.	Which of the following best describes the reproductive characteristics of this species?

a.	Seasonal breeder, gestation of about 164 days, interbirth interval of 360 days
b.	Nonseasonal breeder, gestation of about 164 days, interbirth interval of 380 days
c.	Seasonal breeder, gestation of about 185 days, interbirth interval of 360 days
d.	Nonseasonal breeder, gestation of about 164 days, interbirth interval of 360 days
e.	Seasonal breeder, gestation of about 185 days, interbirth interval of 380 days

Answer:  a.  seasonal breeder, gestation of about 164 days, interbirth interval of 360 days
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 699.   
Task:  T4K6
Species:  Primary (macaque)

44.	If, in the opinion of the attending veterinarian, it is inappropriate for this species to exercise because of their health, condition, or well-being a research facility may be exempted from providing opportunities for such exercise. How frequently and by whom must these exemptions be reviewed

a.	Weekly by a designated husbandry staff member
b.	Weekly by a designated laboratory staff member
c.	Weekly by the attending veterinarian
d.	Every 30 days by a designated husbandry staff member
e.	Every 30 days by the attending veterinarian

 Answer: e. Every 30 days by the attending veterinarian
Reference: CFR (Code of Federal Regulations). 1985. Title 9 (Animals and Animal Products) Subchapter A (Animal Welfare) 3.8 (pg. 51). Washington, D.C.: Office of the Federal Register.
Task:  T4K10
Species:  Primary (dog)

45.	What animal model can be produced in this species by the administration of alloxan?

a.	Hyperthyroidism with hypertrophic cardiomyopathy
b.	Myasthenia gravis with megasophagus and facial nerve paralysis
c.	Huntington’s disease with severe dyskinesias
d.	Renal hypertension and intrarenal reflux
e.	Type 2 diabetes mellitus with severe coronary atherosclerosis

Answer: e.  Type 2 diabetes mellitus with severe coronary atherosclerosis
Reference: Bellinger, DA, EP Merricks, and TC Nichols 2006. “Swine Models of Type 2 Diabetes Mellitus: Insulin Resistance, Glucose Tolerance, and Cardiovascular Complications.” ILAR Journal 47(3): 243-258 
Task:  T9K1
Species:  Primary (pig)

46.	The potential for the transmission of what organism, capable of causing high mortality in one of these species, is the primary reason that these two animal types should be housed separately

a.	Beilerincus rudolphus
b.	Bordetella bronchiseptica
c.	Chlamydophila psittaci
d.	Cytomegalovirus
e.	Pasteurella multocida

Answer:  b. Bordetella bronchiseptica
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 203-247.
Task:  T6K4
Species:  Secondary (guinea pig)

47.	In a colony of immuno-deficient strain of mice, suckling mice were noted to have watery diarrhea and mortality. The histology is presented on the slide. What is the most likely etiologic agent: 

a.	Citrobacter rodentium
b.	Clostridium piliforme
c.	Mouse parvovirus (MPV)
d.	Mouse Hepatitis Virus (MHV)
e.	Mouse Adenovirus (MAdV)

Answer: d – Mouse Hepatitis Virus (MHV)
Reference: Diseases of Research Animals. At the website www.radil.missouri.edu/info/dora
Task:  T1K8
Species:  Primary (mouse)

48.	This animal is used to model: 

a.	Cyclic hematopoiesis
b.	Muscular dystrophy
c.	Hypothyroidism
d.	Copper storage disease (Wilson’s disease)
e.	Beiler’s disease

Answer: d – Copper Storage disease
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 395-459.
Task:  T9K1
Species:  Primary (dog)

49.	What ideal light level is recommended to house this species:

a.	125 lux
b.	325 lux
c.	525 lux
d.	800-1100 lux
e.	> 1400 lux

Answer: d – 800-1100 lux
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 927.  
Task:  T6K4
Species:  Primary (macaque)

50.	The animal pictured is predisposed to which of the following congenital disorders

a.	Schistosomus reflexus 
b.	Sporadic exophthalmos
c.	Factor IX Deficiency
d.	Diaphragmatic Hernia
e.	Hirschsprung’s Disease

Answer: d. Diaphragmatic Hernia
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 309-328. 
Task:  T1K5
Species:  Tertiary (other rodents)

51.	The age of sexual maturation of the animal pictured is:

a.	4-6 months
b.	5-7 months
c.	6-8 months
d.	7-9 months
e.	8-10 months

Answer: b. 5-7 months
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 329-364   
Task:  T4K1
Species:  Primary (rabbit)

52.	The goat in this image is undergoing what procedure?

a.	Dialysis
b.	Plasmapheresis
c.	Terminal exsanguination
d.	Whole blood transfusion
e.	Whole blood donation

Answer: b. Plasmapheresis
Reference: Styer, C. 2006.  “Repeated Automated Plasmapheresis in Goats (Capra hircus): A Clinically Safe and Long-term Refinement to Current Antibody Recovery Techniques.”  JAALAS 45(6): 49-53
Task:  T5K2
Species:  Secondary (goat)

53.	This kidney was removed from a 12-day-old puppy that died acutely.  What is the most likely etiologic agent responsible for the lesions shown in this slide?

a.	Multifocal nephritic necrosis
b.	Canine herpes virus
c.	Canine distemper virus
d.	Focal nephritic abscess
e.	Canine parvovirus

Answer:  b.  Canine herpes virus
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 415.
Task:  T4K1
Species:  Primary (dog)

54.	Several infant mice in your facility had stunted growth and yellow diarrhea. What is the causative agent?

a.	MHV
b.	Pneumonia virus of mice
c.	Reovirus-3
d.	Tyzzer’s disease
e.	EDIM

Answer:  e.  EDIM
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 73-74.
Task:  T1K8
Species:  Primary (mouse)

55.	What is this specialized technique used to track air movement?

a.	Computational flow analysis
b.	Air movement flow analysis
c.	Computational fluid dynamics
d.	Computed fluid diagnostics
e.	Computational flow diagnostics

Answer:  c.  Computational fluid dynamics
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, pp. 920-921.
Task:  T6K5
Species:  None

56.	An investigator at your facility would like to import this animal.  What is the CITES classification for this animal?

a.	I
b.	II
c.	III
d.	IV
e.	V

Answer:  a, Saguinus oedipus (cotton-top tamarin) ,Appendix I
Reference:  http://www.cites.org/eng/app/appendices.shtml
Task:  T7K1
Species:  Tertiary (other NHP)

57.	What is the OLAW reporting period for animal activity at an institution?

a.  Monthly
b.  Semiannually
c.  Every 36 months
d.  Quarterly
e.  Annually

Answer:  e.  Annually
Reference:  Office of Laboratory Animal Welfare.  2002.  Public Health Service Policy on Humane Care and Use of Laboratory Animals.  NIH, p. 17.
Task:  T5K1
Species:  None

58.	What does this condition indicate and what is substance secreted?

a.  Fear;  Red Tears
b.  Eustress; Crusts
c.  Distress; Porphyrin
d.  Stress; Porphyrin
e.  Pain; Crusts

Answer:  d.  Stress, Porhyrin
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 123.
Task:  T2K1
Species:  Primary (rat)

59.	What does the acronym depicted in this slide stand for?

a.  Internal Animal Transfer Association
b.  International Animal Transfer Association
c.  International Animal Transfers to America
d.  International Air Transfers to America
e.  International Air Transportation Association

Answer:  e.  International AirTransportation Association 
Reference:  http://www.iata.org/about/
Task:  T7K1
Species:  None

60.	Which anesthetic agent may provide some post-operative analgesia to these rats?

a.  Tribromoethanaol
b.  Ketamine / Xylazine
c.  Pentobarbital
d.  Halothane
e.  Propofol

Answer:  b.  Ketamine / Xylazine
Reference:  Kohn, DF, Wixson, SK, White, WJ, and Benson, GJ.  1997.   Anesthesia and Analgesia in Laboratory Animals.  Academic Press, New York, NY,  p. 174.
Task:  T2K5
Species: Primary (rat)

61.	There are anatomic structures in this species which can create difficulties during intubation.  Which of the following is NOT an anatomic characteristic of this species?

	a.	Long tongue
	b.	Small oral opening
	c.	Hamuli epiglottici
	d.	Palatal ostium

Answer: d.  Palatal ostium
Reference:  Lipman, NS, Marini, and Flecknell, PA.  1997.   Anesthesia and Analgesia in Laboratory    Animals.  Academic Press, New York, NY,  p. 211.
Task:  T2K6
Species:  Primary (rabbit)

62.	Which of the following restraint methods is considered the least distressful for this species?

	a.	Stanchion
	b.	Restraint sling
	c.	V-trough
	d.	Snout snare

Answer:  b.  Restraint sling
Reference:  Smith, AC, Ehler, WJ, and Swindle, MM.  1997.   Anesthesia and Analgesia in Laboratory    Animals.  Academic Press, New York, NY,  p. 314-315.
Task:  T2K10
Species:  Primary (pig)

63.	How frequently is this room in a research facility to be inspected by a USDA Veterinary Medical Officer?

	a.	Never
	b.	Semiannually
	c.	Annually
	d.	Triennially

Answer:  c.  Annually
Reference:  Animal Welfare Act, Title 7-Chapter 54, section 2.146(a), p. 9.
Task:  T5K1
Species:  Primary (dog)

64.	Which of the following drugs has been associated with the clinical disease depicted in this slide?

a.	Metomidate
b.	α-Chloralose
c.	Chloral hydrate
d.	Tiletamine
e.	Methohexital

Answer: c. Chloral hydrate
Reference: Fox, J., Anderson, L., Lowe, F., and Quimby, F. 2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 959.
Task 2:  T2K7
Species:  Primary (rat)

65.	According to the AVMA Panel on Euthanasia, which of the following is an acceptable method of euthanasia for the animals depicted in this slide?

	a.	Hypothermia
	b.	Nitrogen
	c.	Carbon dioxide
	d.	Barbiturates

Answer: d.  Barbiturates (b is conditionally acceptable)
Reference:  Beaver, B., et.al.  2001.  2000 Report on the AVMA Panel on Euthanasia.  JAVMA 218 (5) p. 688, 693, and 696.
Task:  T5K2
Species:  Primary (mouse)

66.	If the mean is 12 and the standard deviation is 3, what is the range that accounts for 95% of the values on this plot?

	a.	0 to 25
	b.	9 to 15
	c.	6 to 18
	d.	3 to 21

Answer:  c.  6 to 18 (two standard deviations from the mean account for 95% of the values)
Reference:  http://en.wikipedia.org/wiki/Normal_distribution
Task:  T10K3
Species:  None

67.		In this species, what is the purpose of the lateral line (identified by arrow)?

a.	Detection of odor
b.	Detection of water current
c.	Detection of light
d.	Detection of flavor

Answer:  b.  Detection of water current
Reference:  Fox, J., Anderson, L., Lowe, F., and Quimby, F.  2002.  Laboratory Animal Medicine, 2nd ed.  Academic Press, San Diego, CA, p. 809.
Task:  T2K2
Species:  Secondary (Xenopus)

68.	Which of these diagrams represents a latin square design?

a.	A
b.	B
c.	Neither
d.	Both

Answer: a.  A
Reference:  Shott, Statistics for Health Professionals, 1990; http://bill.psyc.anderson.edu/exdes/ex8.htm 
Task:  T10K1
Species:  None

69.	The primary function of the equipment depicted in the slide is:

	a.	Non-invasive measure of airway parameters, resistance and conductance
	b.	Chronic intravenous infusion
	c.	Metabolic chamber to measure urine and fecal output.
	d.	Measure blood pressure (indirect)

Answer: a. Non-invasive measure of airway parameters, resistance and conductance 
Reference:  Unrestrained Whole Body Plethysmograpy - Buxco Research Systems (www.buxco.com)
Task:  T5K2
Species:  None

70.	The purpose of the equipment shown in this slide is to:

a.	 elicit a tactile startle response (stop movement)
b.	 measure foot pad swelling in rodents
c.	 measure body temperature via foot pad
d.	 measure mechanical stimulation and sensory threshold in rodents

Answer: d. measure sensory threshold in rodents
Reference:  Mulder GB, Pritchett K. 2004. “Rodent Analgesiometry: The Hot Plate, Tail Flick and Von Frey Hairs.” Cont Topics in Lab Anim Med 43 (3): p. 54-55.
Task:  T3K1
Species:  Primary (rat)

71.	What is the schedule assigned to the controlled substance (combination drug) depicted in this slide?

	a.	Schedule I
	b.	Schedule II
	c.	Schedule III
	d.	Schedule IV
	e.	Schedule V

Answer:  c.  Schedule III
Reference:  www.virbacvet.com/product_guide/euthanasia.html
Task:  T7K1
Species:  None

72.	The device depicted in this slide can be used to teach all of the following EXCEPT:

	a.	Intratracheal intubation
	b.	Intraperitoneal injection
	c.	Blood collection
	d.	Intracerebral cannulation

Answer:  d.  Intracerebral cannulation
Reference:  http://www.braintreesci.com/MD-PVC.htm
Task:  T8K2
Species:  Primary (rat)

73.	Which of the animals depicted in this slide are covered by the Animal Welfare Regulations?

	a.	Animal A
	b.	Animal B
	c.	Animal C
	d.	Animal D
	e.	All are exempted

Answer:  c.  Animal C
Reference:  CFR (Code of Federal Regulations). 1985. Title 9 (Animals and Animal Products) Subchapter A (Animal Welfare) 1.1 Definitions (p. 19). Washington, D.C.: Office of the Federal Register.
Task:  T7K1
Species:  None

74.	All of the following are appropriate endpoint criteria for animals on studies similar to the one depicted in this slide EXCEPT for:

	a.	Weight loss
	b.	Tumor mass
	c.	Presence of ulcer
	d.	Impairment of ambulation

Answer:  a.  Weight loss
Reference:  Ullman-Cullere, M. H. and C. J. Foltz.  1999.  “Body Condition Scoring: A rapid and accurate method or assessing health status in mice.”  Laboratory Animal Science.  49(3): 319-323.
Task:  T2K10
Species:  Primary (mouse)

75.	According to the Animal Welfare Regulations, if doing surgery on the animal depicted in this slide, what is required?

	a.	Dedicated surgical suite
	b.	Use of aseptic technique
	c.	Sterile surgeon gown
	d.	Ethanol sterilized instruments

Answer:  b.  Use of aseptic technique
Reference:  CFR (Code of Federal Regulations). 1985. Title 9 (Animals and Animal Products) Subchapter A (Animal Welfare) 2.31(d)(ix) (p. 34). Washington, D.C.: Office of the Federal Register.
Task:  T7K1
Species:  Primary (rat)

END OF EXAM